Composite

Part:BBa_K1675015:Design

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-12)


J23119+B0034+NhaA+J23119+B0034+NhaB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 324
    Illegal NheI site found at 1245
    Illegal NheI site found at 1268
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 521
    Illegal BglII site found at 1304
    Illegal BamHI site found at 1058
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 384
    Illegal AgeI site found at 2302
    Illegal AgeI site found at 2614
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mutation of CTG to CTA Leu.(site:1552bp); mutation of GCT to GCG Ala.(site:1630bp); mutation of ACC to ACT Thr.(site:1780bp); mutation of GAC to GAT Asp.(site:1786bp); mutation of ACC to ACT Thr.(site:1891bp); mutation of GTA to GTG Val.(site:1906bp); mutation of GCA to GCG Ala.(site:2254bp); mutation of GAG to GAA Glu.(site:2281bp); mutation of GTC to GTA Val.(site:2713bp)


Source

The gene NhaA and NhaB come from Escherichia coli str. K-12. The J23119 promoter and the B0034 RBS come from the standard parts.

References